7°C and the bottom to 36. the reader’s stated range. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. Real-time experimental control (REC) that automatically activates a procedure as soon as a specific data value is reached. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. 2 only) SpectraMax M-series Microplate readers. 1. 0) OD (500 to 1100) RFU (5. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. The Molecular Devices Gemini EM is built on the Gemini platform delivering high quality top and bottom reading for both solution and cell based assays. DNA quantification with fluorescence dyes. plate over and over again. 25 fmol/well 384-well plate) Filters/Mirrors: Fluorescein 1 pM typical (0. Endpoint, kinetic, spectrum. A solution for low volume applications. SoftMax Pro. Features. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Figure 6: Transcreener ADP2 FI assay principle. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. TRF as Secondary Detection Mode: TRF is supported as a secondary detection mode by these readers: Gemini XS, EM, XPS, M2, & FlexStation. Equipment Details. LVF Monochromator™ : Excitation and Emission. Microplate Readers Molecular Devices Spectramax iD3 Microplate Reader. Plate stacker and robot integration The SpectraMax M2 and M2e readers can be integrated with the A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The SpectraMax 190 reader can be integrated with Molecular . Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. $16,000. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Perkin Elmer 1420-2560 Victor X Series Plate Reader. Tristar 3 Multimode Microplate Reader. SpectraMax M5 is a fantastic plate reader - easily my #2 behind PerkinElmer (if you can afford them). It features a modular design that allows users to add or change detection cartridges according to their needs. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. We here describe a fluorescence resonance energy transfer (FRET) based fluorescence plate reader assay to monitor the interaction between fluorescently tagged sortilin and p75 NTR in live cells. SpectraMax M2 and M2e Multi-Mode Microplate Readers User Guide Molecular Devices SpectraMax M2 and M2e Multi-Mode Microplate Readers. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Products : SpectraMax M2/M2e reader, SpectraMax. BioTek Synergy HTX Microplate Reader - Multi Mode (Abs + FL + Lum), Bio-tek, Used plate reader, BioTek multi-mode detection, PMT for luminescence detection, kinetic assays, Time Resolved Fluorescence, Gen5 software, absorbance reader, fluorescence detection, used lab. STAKMAX. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR. Refurbished. spr * SpectraMax i3(x) SpectraTest ABS2 v1. Wide range of applications. 020 OD 2. SpectraMax ® M2 Multi-Mode Microplate Reader. Multimode Readers Comparison Chart. In the presence of ACE, the PL intensity was increasing by time, while the PL intensity sustained a low and stable fluorescence in 3 hours in the absence of ACE. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13Fluorescence Microplate Readers. 314 low lamp level warning. S. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide. 53 - 60 kg, (117-132 lb), depending on configuration. It is particularly powerful when monitoring fast kinetic assays in a high. Add to Cart. The plate blank’s RFU values should be subtracted from the RFUs of all samples of the plate. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. Plate chamber design of the SpectraMax category 1 microplate readers. SpectraMax Quant dsDNA Assay Kits. Ideal for a wide range of applications, including Western Blot analysis, this SpectraMax i3x includes the additional MiniMax 3000 Imaging Cytometer module for advanced cell imaging. An assay plate containing a 15-point standard curve was read on the SpectraMax. Whether fluorescence, chemiluminescence, or visible detections - select the optimal plate for your assay!If your microplate reader requires an adapter for top reading (e. The flexibility of a high end multimode plate reader with environmental control. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. on SpectraMax M2 fluorescence plate reader (Molecular Devices, CA, USA): excitation wavelength 320nm, emission wavelength 470nm. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. Tecan’s. ZERO BIAS - scores, article reviews, protocol conditions and moreFluorescence Filter with holder 625nm BW 35nm (Molecular Devices cat. Description The onboard microprocessor calculates and reports the absorbance, % transmittance, and/or fluorescence for each well of a microplate or from the cuvette port. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. the reader’s stated range. Add to Cart. The plate is set up for reading and images are acquired according to specified parameters. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. A microplate reader asPython 3. Microplate readers’ standard detection modes. D 59 A. Reads various kinds of 96-wells plates, and is equipped with shaking function. The SpectraMax i3x reader provides theThis Molecular Devices SpectraMax iD5 Microplate Reader is made in Oct. For example, microplates containing different samp les can be read using the same orThe plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). Therefore, ADP production is proportional to an increase in fluorescence. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Molecular Devices SpectraMax™ M2e Microplate Reader w/ Warranty. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. The SpectraMax i3 Multi-Mode Plate Reader enables characterization of proteins by measuring intrinsic tryptophan fluorescence. Figure 2 shows a Lck kinase dilution curve obtained using the FAM-p34cdc2-derived peptide. SpectraMax ABS Plus SpectraTest ABS2 v1. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and. Item Description Sensitivity 10amol/wellalkalinephosphate200µL Wavelengthrange 250-850nm Crosstalk <0. 2. Plate reader settings for NanoOrange assay. Plate reader set to 50 reads. Reviews. SoftMax Pro GxP, together with a Molecular Devices plate reader and Molecular Devices GxP Admin software, provides a fully integrated hardware and. The Spectr. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. A whole well fluorescence imaging system that can collect information from all cells inside the well, from 6- to 384-well formats. 0 out of 5 $ 9,995 $ 9,495. 1. D. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one systemFAST AND ACCURATE METERTECH Microplate Reader $3,650. The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. SpectraMax M2 Multi-mode Plate reader with Absorbance and Fluorescence detection, Includes computer with software, IQ validation, and warranty. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. This enables the standard curve to be plotted and sample concentrations to be calculated automatically by SoftMax. 67. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell. USING OLIGREEN REAGENT IN GEMINI XS, GEMINI EM AND SPECTRAMAX M2 MICROPLATE READERS Step 6: Add 100 µL of each concentration of oligonucleotide to the wells of the microplate in triplicate. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. The opaque walls prevent stray light from being detected from neighbouring wells for best accuracy. Danaher Inc spectramax m2 fluorescence microplate reader Spectramax M2 Fluorescence Microplate Reader, supplied by Danaher Inc, used in various techniques. Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. BioTek Synergy 2. Type Clear: Add to Cart. Microplate readers’ standard detection modes are. We use Spectramax M5 microplate reader in our lab. SpectraMax Spectrofluorometers. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. BMG Labtech - THERMOstar. 87. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. min at 37°C. The chamber. 67. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for. The following general steps are taken with our absorbance and fluorescence readers. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. com Setup for Omnia® assays on SpectraMax®. SpectraMax Microplate Readers. Shop all used microplate readers and parts from a variety of manufacturers. It combines the sensitivities of filters with the convenience of a monochromator to provide the complete laboratory solution with absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. Fluorescence measurements no longer require you to adjust the gain. Brand: MOLECULAR DEVICES. KEY FEATURES • Use the touchscreen to easily set up methods, runSpectraMax® i3xMulti-ModeMicroplateReader SpectraMax® i3Multi-ModeMicroplateReader FlexStation® 3Multi-ModeMicroplateReader SpectraMax® M5eMulti-ModeMicroplateReader SpectraMax®M5Multi-ModeMicroplateReader SpectraMax® M4Multi-ModeMicroplateReader SpectraMax® M3Multi. Step 4: Use the Template Editor to assign wells to ‘Standards’ and ‘Unknowns’ groups (Figure 2). 100 readings per well gives optimal Z’ values for this assay. SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence and luminescence. 020 OD 2. Luminescence, and Fluorescence microplate re aders—providing extensive data calculation. CA$14,500. Tecan Infinite F200 Fluorescence Microplate Reader. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. Protein assays. Part Number Description 1-2100-0548 USB computer connection cable, 3 meter (9. WinKQCL ® Software guides the user through the process of setting up a microplate template and running an assay to determine the amount of endotoxin in a sample solution. Detection Ranges: absorbance 200-1000nm, fluorescence Ex: 250-850nm / fluorescence Em: 360-850nm, luminescence: 360–850 nm. For multiplexed luminescent and fluorescent assays, using a white plate will support maximum light output signal for the luminescent portion of the assay, but result in higher crosstalk and background for the fluorescent portion of the assay. SpectraMax M2 and use thereof is covered by issued (U. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. The REF button has no valid meaning for fluorometric use, and if pressed when in fluorometric mode, will switch. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (plate reader can measure multiple readouts, including UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Wide range of applications The VersaMax reader covers a wide range of applications:Molecular Devices SpectraMax M2 Multimode Plate Reader. Analyze the dataHere we report the use of the EarlyTox ™ Live/Dead Assay Kit in combination with SpectraMax ® fluorescence microplate readers (Figure 1). 1) Select the correct plate and the wells that you want to measure. B. SpectraMax M2E Multimode plate reader with cuvette option, ABS and FLR reading and extended range. From advanced multimode luminescence, fluorescence, absorbance, BRET and FRET capabilities to dedicated microplate or single-tube luminometers, there is a GloMax® system for every lab and. The SpectraMax iD5 reader rapidly performs absorbance measurements that are used to plot standard curves and calculate sample concentrations using a preconfigured protocol in SoftMax Pro Software. Flexibility to read 6- to 384-well plates. The SpectraMax iD3 reader measures absorbance, fluorescence, and luminescence. With-dye, Continual Mode Assay on a SpectraMax M2 Plate Reader. 1 cm) Weight 27 lb. Step 2: Click SoftMax Pro’s Read button. 3: The cuvette chamber. Filters: Excitation and emission slides for 4 filters each. Customer Satisfaction is our number one goal All lab equipment in stock already tested and ships in 1-3 days The plates were then read with the SpectraMax M3 plate reader (Molecular Devices, Sunnyvale, CA) to obtain absorbance readings at 570 nm. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. It allows you to explore cellular pathways and protein activation and expression in one system. Absorbance (monochromator). Screen Snapshot of Settings Dialog Box As the ratio of ADP:ATP increases, the proportion of bound tracer vs. 0 × 107) RLU 0. Multimode microplate readers deliver both performance and economy. 0 out of 5 $ 7,999. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2. 4. SPECTRAmax GEMINI’s wavelength choices for cutoff filters are 420, 435, 455, 475, 495, 515, 530, 550, 570, 590, 610, 630, 665 and 695 nm. Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. Automated live cell imaging – cell counting and confluence. 119. The LVis Plate contains 16 spots which can be loaded with 2 microliters of sample for subsequent absorbance measurements. 5008531B 3 Contents Chapter1:SpectraTestMulti-ModeValidationPlateOverview 4 Multi-ModeProtocols 6 Chapter2:GettingStarted 8 CertificateofCalibration 8SpectraMax M2. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The pre-configured protocols can be selected for each assay and. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). In addition, the SpectraMax iD5Popular answers (1) If you are going to read the plate from the top, you should use a solid black plate. The cuvette must be in place when you read the plate. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). ~ Bio-Tek ELx808 Microplate Reader $2,795. Microplate Readers Molecular Devices SpectraMax M2 Multimode Plate Reader. Search: Submit search Search filters Search This site Search Cornell. If something isn't quite right, all three reliable fluorescence and luminescence plate readers can tell you before. Temperature control range from Ambient +4º to 50ºC. Allows you to choose your sample format depending on your unique research and throughput needs. Molecular devices SpectraMax M5 Pdf User Manuals. For more sample throughput, the system canProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190. The SpectraMax microplate readers support a wide variety of assay categories including . The SpectraMax® ABS Plus Microplate Reader can run . 6% for the on-board optics (monochromators). Type Clear: Add to Cart. Test Plates for absorbance and fluorescence intensity provide a complete traceable solution for validating optical performance of the SpectraMax M2 reader, automatically. 7. I've got a LT4500 microplate reader and was hoping to digest the pellets in papain overnight, simply mix a measure of that sample with DMMB solution in a 96 well plate and read absorbance at 525nm. This is a result of the light being attenuated and scattered by the plastics of the well-bottom which can. 315 can't find zero order. 2 % of reading SpectraMax Plus384 microplate reader and absorbance plate ABS1 SpectraMax ABS Plus microplate reader and Absorbance plate. The FlexStation 3 reader’s optics are designed to easily adapt to changes in assay requirements while maintaining the optimal performance of a single-mode reader. These measurements can include parameters such as absorbance, fluorescence intensity and. Fill a clean plate with water. 5,112,134 5,766,875. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). Dual monochromators allow selection of any absorbance wavelength between 200 nm and 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. . Multi-Mode Microplate Readers including the M3, M4, M5, and M5. Because. 2018. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Temperature control (RT+3°C – 42°C) Liquid dispensers with reagent heater and stirrer. S. Prior to fluorescence analysis, the lid from the OmniTray plate was removed. a multi-detection microplate reader with two-mode cuvette port Spectramax® m2m2e user guide SpectraMax M2e user guide cover 1 1 42106 9:53:28 AM SpectraMax M2 SpectraMax…Spectramax i3 Plate Reader. Settings for SpectraMax iD3 Multi-Mode Microplate Reader. Biotek FLx800 Fluorescence Microplate Reader. the reader’s stated range. (30. When the user applies a set temperature of 37°C, the top is heated to 37. U. Microplate Readers Include the M3, M4, M5, and M5. The SpectraMax® M2 and M2e Systems from Molecular Devices are multi- detection microplate readers with dual- monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading. 020 OD 2. 3. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for wavelength selection was compared to that using a filter pair specific for the Alexa594 tracer. The assay is carried out in a standard 96-well plate. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. FAST AND ACCURATE METERTECH Microplate Reader $3,650. For a complete list of the instruments supported by this release of theThe HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). White plates reflect the light, enabling even low levels of Luminescence to be detected. In simplest terms, a plate reader measures and records a signal produced from the wells of a multiwell plate. 2. The Infinite® M1000 from TECAN is a high-end multimode Microplate Reader with premium Quad4 Monochromators. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. BMG Labtech - THERMOstar. The GloMax® line includes high-performance plate readers with a range of detection options. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. Perkin Elmer EnVision 2104 Multilabel Plate Reader, Perkin Elmer, Envision Plate Reader. Stage and carriage are homed and position is tested and verified. The plate reader. Peeking into a research lab today, it would be rare to see a line-up of microplate readers on a bench, each capable of performing only a single type of measurement. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Important things to consider when purchasing a multimode microplate reader. The SoftMax Pro Software validation package and IQ/OQ validation protocols include tools for FDA 21 CFR Part 11 compliance. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. Plate reader. . I have been using the Molecular Devices SPECTRAmax GEMINI XS for the last 6 months, mostly in time-resolved fluorescence,. The first step consisted of taking an initial fluorescence measurement using Molecular Devices SpectraMax® M2 fluorescence plate reader of a 96-well plate containing PET-RAFT polymerization reaction mixtures in individual wells. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). 60 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. Last lab had ours for years and never had a problem with it. However, proNGF-induced receptor complex formation has been difficult to directly assess other than by western blotting. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators. Fig 2: Enzymatic response of synthetic compounds. LuminescencePhotometricPerformance. $ 9,499. Optical System: High energy xenon flash lamp. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. We would like to show you a description here but the site won’t allow us. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax ® Pro. SpectraMax M2 and SpectraMax M2e from Molecular Devices are multi- detection microplate readers with dual-monochromators, dual-mode cuvette ports and top- and bottom-reading capability (top-reading only on SpectraMax M2). The protocol contains settings for reading the plate, as well as template groups that allow a user to easily set up analysis, including calculation. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). It is typically used to determine the concentration of nucleic acids and proteins. . 49 µM,. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. Many of the following instruments are multimode readers that, in addition to luminescence detection, can also be used to measure UV. 25 to 3. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck® Sensor. When a. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. Microplate Readers Include the M3, M4, M5, and M5. Optimal excitation APPLICATION NOTE and emission wavelengths for SpectraMax M5 fluorescence. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Model: SpectraMax M2. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. Multimode Microplate Readers. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and moreThe SpectraMax iD3 reader measures absorbance, fluorescence, and luminescence. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400–750 nm for readings in fluorescence polarization mode (M5, M5e models only). Main Menu Toggle. 312 gain calibration failed. spr SpectraMax iD5 SpectraTest ABS2 v1. Appendix. Temperature Control: ambient from +4 to 45C. Data from slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Compact and tunable absorbance microplate readers DATA SHEET. Description 1. E Models. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Cells in each image are identified by SoftMax Pro Software and cell-by-cell fluorescence intensity, time-resolved fluorescence (M4, M5, M5 e models only) or wavelength-selectable luminescence modes, and 400 750 nm for readings in fluorescence polarization mode (M5, M5 e models only). 0 × 103 to 2. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize. Screen Snapshot of Settings Dialog Box As the ratio of ADP:ATP increases, the proportion of bound tracer vs. Two important settings to obtain the best results in TRF assays. 7. UT-6550 Microplate Reader is a reliable and robust instrument for a wide. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. Absorbance (monochromator) Fluorescence (monochromator; FI, TR, FP) Luminescence; Molecular Devices Spectramax M2. The Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. Set up a plate section for a second emission scan as in Step C above, except for the added Emission Cutoff Filter. A). 00. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Usage/Application: Lab. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. 0 out of 5 $ 3,895. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. With optimized reagents, option to operate the readers. Biotek Elx800 Microplate Reader. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. You should determine the expected mean OD value by reading a clean water-filled plate from each new lot. Standard features include a cuvette port,. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. A Costar® 24-well tissue culture plate with col1 = blanks and doubling cell. Parameter EarlyTox Live/Dead EarlyTox Glutathione EarlyTox Caspase-3/7 R110 Read mode Fluorescence Fluorescence Fluorescence Read type Endpoint Endpoint Endpoint Wavelengths Lm1:. SpectraMax ® M2 Multi-Mode Microplate Reader. The fluorescence was measured in the SpectraMax M3 Microplate reader. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Read the microplate Step 1: Place the microplate in the reader. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. D 4/21/06 9:53:29 AM . Thermo Fluoroskan Ascent FL Fluorescence Microplate Reader. Molecular devices SpectraMax M3 Pdf User Manuals. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. Perkin Elmer Envision. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The software is easy to use and contains settings for hundreds of commonly used assays, such as BCA and MTT. orporation. For a higher degree of automation, the Automation. View online or download Molecular devices SpectraMax M3 User Manual. Next add 100 µl of workingThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. If a lab requires instruments to read ELISA plates, one to do fluorescence and one for luminescence, it will likely look for one instrument that can handle all these. 00 USD. Page 28 Chapter 5: Read Modes and Read Types 1. patent nos. 627. (a) Chemical structures of 1 (HADP) and its analogues 2–4. 306 plate air cal fail,low light. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. The UV-Vis SpectraMax 190 Microplate Reader for Quantification of DNA and endpoint, kinetic, and spectral scanning analyses. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. Insert the snorkel into the bottle and then insert the snorkel into the snorkel clamp. Category 2 and 3 microplate readers use gradient heating as well. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2. On the SpectraMax iD5 reader, install the 320/100 excitation filter and 616/10 and 665/10 emission filters, and then select these filters from the Excitation and Emission dropdown wavelength menus. The SpectraMax M2e is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. The kit includes everything you need: reagents, buffers and a microplate. Add to Cart. for the plate. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Absorbance cuvette port. 209, but in 96-well plate was 0. In the second assay, HepG2 cells were plated into a 96-well clear-bottom, blackwalled plate at 10,000 cells/well and incubated overnight at 37°C. Instruments are configurable for the assay-specific detection modes and performance your laboratory needs. SpectraMax M2. . The SpectraMax® M2 and M2e Multimode. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and.